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  • GM Food Extraction, Amplification, and Electrophoresis Kit with GelGreen™ and 0.2-mL Tubes (with prepaid coupon)

GM Food Extraction, Amplification, and Electrophoresis Kit with GelGreen™ and 0.2-mL Tubes (with prepaid coupon)

Item # 211372 Exclusive This product is exclusive to Carolina Biological Supply. With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.

Explore a modern application of genetic engineering through this exciting laboratory activity, which investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and genetically modified (GM) plant material (provided controls), and from food products of their choice. Kit includes materials for 25 PCR reactions.
Price

$259.85

In stock and available to ship.
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Overview

Advanced - For experienced high school and college classes; requires some basic technical skill.

Explore a modern application of genetic engineering through this exciting laboratory activity. Many commercial crops are genetically modified (GM) to include a transferred gene (transgene) for herbicide resistance. This activity investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and GM plant material (provided controls), and from food products of their choice. They use the extracted DNA as a template in 2 separate PCR reactions run under the same conditions. One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification. Each group works with a plant control and a food product. Kit includes materials for 25 PCR reactions and a prepaid coupon to request perishable materials later at your convenience. Contact us or return coupon to request delivery of perishables. Note: Order kit to arrive 1 month before performing lab.

FREE 1-year access to digital resources that support NGSS instruction included.
  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.

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What's Included

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Wild-Type Soybean Seeds 16 Included
Roundup Ready® Soybean Seeds 16 Included
pBR322/BstN1 Markers, 130 µL 1 Included
Tris/EDTA (TE) Buffer with RNase A, 3 mL 1 Included
35S Primer/Loading Dye Mix, 500 µL 1 Included
Tubulin Primer/Loading Dye Mix, 500 µL 1 Included
Edwards' Buffer, 20 mL 1 Included
Isopropanol, 100%, 10 mL 1 Included
Agarose, 8 g 1 Included
PCR Tubes Containing Ready-To-Go-Beads™ (0.2-mL capacity) 25 Included
Mineral Oil, 5 mL 1 Included
Pellet Pestles 15 Included
Microcentrifuge Tubes 25 Included
TBE 20x, 150 mL 1 Included
GelGreen® Nucleic Acid Stain, 100 µL 1 Included
5 M NaCL, 8 mL 1 Included
Latex Gloves 12 Included
Staining Trays 6 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Document Camera or Overhead Projector Needed, Not Included
Planting Pot or Flat Needed, Not Included
Potting Soil Needed, Not Included
Soy or Corn Food Products Needed, Not Included
Micropipets and Tips (1 to 1,000 µL) Needed, Not Included
Microcentrifuge Tube Racks Needed, Not Included
Microcentrifuge (for 1.5-mL tubes) Needed, Not Included
Thermal Cycler Needed, Not Included
Water Bath or Heating Block Needed, Not Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
UV or Blue Light Transilluminator Needed, Not Included
Permanent Markers Needed, Not Included
Container of Crushed Ice Needed, Not Included
Camera (optional) Needed, Not Included
Vortexer (optional) Needed, Not Included
Roundup® Herbicide (optional) Needed, Not Included

Resources

How-To & Techniques

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DNA Learning Center

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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