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qPCR Lab: Principles of Quantitative PCR

Item # 216326 Online Only This product is not available in our print catalog.

IntermediateEasy to perform; requires some background knowledge.

Students perform a modified version of a quantitative polymerase chain reaction (qPCR) in this hands-on activity. The reaction is modified so that students can observe the change in fluorescence using low-cost equipment and without the need for a qPCR machine. This activity is an excellent illustrative learning tool.

Price

$124.00

Ships from the manufacturer and will arrive on or about 11/10/20

Watch the PCR product glow—without the need for gels!

IntermediateEasy to perform; requires some background knowledge.

In this hands-on activity, students perform a modified version of a quantitative polymerase chain reaction (qPCR). qPCR monitors the amount of PCR product produced in real time using fluorescent dyes or probes. This lab modifies the reaction so that students can observe the change in fluorescence firsthand using low-cost equipment and without the need for a qPCR machine. It is an excellent illustrative tool for learning how fluorescence and PCR can be used to quantify nucleic acids while also providing an introduction to the process of PCR in general. During the activity, students have the opportunity to practice hands-on skills such as micropipetting, PCR, qPCR, and gel electrophoresis (optional). This activity can be completed in one 120-minute block or extended into 3 class periods. Kit contains enough reagents for 8 lab groups (32 students). Note: These reagents require frozen storage. Shelf life is 12 months when stored at -20° C.

  • This item is only available at Carolina.com.
 
Components Qty Included?
2x qGRN Master Mix 1 Included
qPCR Lab Primer Mix 1 Included
DNA Sample 1 Included
Nuclease-Free Water 1 Included
Load-Ready DNA Ladder, 100 bp 1 Included
PCR Strips, 8 Tube 1 Included
6x DNA Loading Dye 1 Included
Digital Student Guide (download from miniPCR website) 1 Included
Digital Instructor's Guide (download from miniPCR website) 1 Included
P51™ Molecular Fluorescence Viewer (item #216321) (or blue light transilluminator; 1 per lab group) Needed, Not Included
miniPCR® or Conventional Thermal Cycler 1 Needed, Not Included